Configure an analysis manually

Before you start

Have measured assay data and a justified procedure for mapping, units, dilution, model and suitability. If you want to practice first, use one of the synthetic walkthroughs. The example settings are not recommendations for an unknown assay.

Work through the configuration

1. Open New analysis. Select Relative potency to compare preparations with a reference, or Concentration determination to estimate unknown concentrations from standards. Choose Configure manually.
2. Use Choose file, check Data selection, and Continue to Map the plate. Assign used wells, levels and replicates as described in Import and map assay data.
3. Continue to Define the dilution series. Set Concentration unit. For relative potency, set Initial concentration and Dilution factor for each preparation and inspect the mapped levels. For concentration work, define the standard concentrations, unknown and quality-control dilution factors, replicate treatment and expected QC concentration when applicable. Inspect Mapped observations for the values that will actually be used.
4. Continue to Model. Choose the exact model card and inspect its settings. Set scientifically justified transformations, weighting, response units, orientation and any model-specific choices that are shown. Options vary by family and model; do not select a model solely because its curve looks better.
5. Check any configured suitability rules and their limits. Add only criteria specified for your use. No user-defined criteria does not mean that an assay has been validated or automatically accepted.
6. Use Back and Continue to inspect the full configuration. Wait for Draft saved and resolve validation messages before Run analysis. Check the filename and settings when you reopen a draft.

Understand saved and unsaved work

The editor saves drafts automatically; there is no separate final Save draft step. Saving draft… or Unsaved changes · saving shortly means your latest changes are still being saved. Draft saved confirms the current saved draft.

Keep or bookmark the draft address after it has saved. Reopen it in the same account and workspace. Workspace selection preserves its own unfinished work; check that the reopened filename and settings match the task you intended to continue.

Configure manually from assisted setup begins separate manual work. The conversation and its saved files remain in the assisted setup; unsent text and unuploaded files are not transferred.

Resolve an invalid or conflicting draft

If a required value is missing or invalid, correct the named field and review the affected mapped observations. Do not substitute zero for unknown units or missing concentrations.

If Draft changed elsewhere appears, another tab or session has changed the saved draft. Inspect the displayed problem and preserve any needed local notes before using Discard local edits and reload saved draft. Check the reloaded values before continuing.

If Draft save failed appears, use the displayed retry or recovery control and confirm Draft saved. Keep the tab open until your changes have saved.